Application

This Anti-Integrin α6 Antibody, clone MA6 is validated for use in FC, IP, IH for the detection of Integrin α6.

Immunoprecipitation, flow cytometry, immunohistochemistry in frozen tissue sections. Product does not detect alpha 6 in Western blot since the epitope most likely involves both alpha 6 and beta 1 integrin subunit. Product does not block alpha 6 integrin mediated cell adhesion to laminin. Activation of integrin signaling properties by MA6 has not been characterized. Immunoprecipitation of VLA-6 from lysate of 10E6 cell equivalent requires 2 µg of MA6. Recommended concentration for flow cytometry - 10 µg/mL, staining of frozen tissue sections - 20 µg/mL.

Optimal working dilutions must be determined by end user.

Cell lines tested by using MA6 to express (6 integrins include: mouse bone marrow-derived mast cells, melanoma cell line B16F1, squamous carcinoma SCC-VII, fibroblast cell line NIH-3T3, splenic T lymphocytes, thymocytes and various T cell lines.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Liquid in 0.02M Phosphate buffer, pH 7.6, with 0.25M NaCl and 0.1% sodium azide.

Format: Purified

Specificity

Reacts with the laminin receptor alpha-6beta-1 and alpha-6beta-4 complexes.

Storage and Stability

Store at +2-8°C for up to 12 months.